By reading this application note, you will learn how to determine the sulfite content present in the beer samples using METTLER TOLEDO’s EasyPlus UV/VIS spectrophotometers.
Sulfur Dioxide (SO2): An Antimicrobial and Antioxidant Agent
SO2 is produced in small quantities as a byproduct of fermentation and added to beverages in the form of sulfite. Sulfiting agents like sodium sulfite, sodium bisulfite, and sodium metabisulfite are used in the food and beverage industry.
Why Determine the Sulfite Content in Beverages?
The European Commission’s (EU) Scientific Committee on Food (SCF) has permitted daily intake of sulfite and its equivalents up to 0.7 mg/kg body weight per day. According to the EU, if the food or beverage contains a total SO2 of more than 10 mg/L then it must be labeled. Sulfite can cause an allergic reaction in some individuals. Therefore, it is necessary to determine the sulfite content in beverages.
How Can the Sulfite Content in Beer Be Determined?
The amount of sulfite content in the beer samples can be easily determined using METTLER TOLEDO’s EasyPlus UV/VIS spectrophotometers. Sulfite binding with p-rosaniline dye in the presence of hydrochloric acid and formaldehyde produces a colored complex. The intensity of this complex is proportional to the amount of SO2 present in the sample which can be determined by measuring the absorption at 550 nm.
This application note includes information on:
- Sample preparation and procedures
- Chemistry
- Solutions and chemicals
- Instruments and accessories
- Results and discussion
- Remarks
Download our free application note to learn more and benefit from our experience in determining the sulfite content in beer.
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