The application provides a method for formazin turbidity measurement. The samples analyzed are ground water and white wine at 860 nm using METTLER TOLEDO UV/VIS spectrophotometer.
Background
Turbidity is commonly measured using nephelometers, i.e. instruments measuring the scattered light of a sample at an angle of 90°. Turbidity measurements performed with spectrophotomers are sometimes referred to as absorbtometric or attenuation turbidity, are useful to indicate relative values or to monitor the turbidity change. Spectrophotometers measure light transmission rather than light scattering using a narrow, short-wavelength light source. The inconsistency in transmission due to the different particle size in solution may lead to subsequent variation in result. Therefore formazin turbidity obtained from UV Vis spectroscopy is instument specific and can be used for relative studies of the sample.
The reaction of hexamethylenetetramine with hydrazine sulfate produces formazin; a heterocyclic and a well-known primary standard for turbidity measurements
This application note includes:
- An introduction to the topic
- A list of instruments and accessories
- An explanation of the method in detail
- Results and remarks
