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The term "water testing" is used to describe diverse procedures to analyze water quality and safety. UV Vis spectroscopy has been used for water testing, and proven to be reliable, fast and easy-to-perform, requiring minimal bench space and able to measure down to low detection limits with sophisticated yet affordable instruments. By this method, a sample of analyte in water can react with given reagents through mixing; after the reaction, the quantity of certain chemicals in the mixture can be determined photometrically with a UV Vis spectrophotometer.
Mettler-Toledo collaborates with MilliporeSigma to deliver solutions to water analysis in industry segments such as Pharma, Biotechnology, Chemicals, Food & Beverage (F&B), Environmental Testing and Power Generation. The solutions can measure more 180 parameters of substances in waste, process and drinking water for safety and quality control. The photometric water tests are based on both test tubes and cuvettes; however, for high throughput sample demands, water testing workflow can be automated. In addition, the guide illustrates the procedures from water sample preparation to results with predefined water application methods for parameters such as COD, Ammonium, Phosphate, Hardness, Nitrate, Sulfate, etc. There are also options to customize water testing methods to fulfill individual needs.
*Spectroquant® is a trademark of Merck KGaA, Darmstadt, Germany
Table of Content
1. Introduction
1.1 Overview of water testing
1.2 UV/VIS Excellence with Spectroquant® test kits
1.3 Capabilities of METTLER TOLEDO UV/VIS Excellence spectrophotometers for water testing
1.4 Overview of water testing methods
2. From Sample Preparation to Result
2.1 Sample preparation
2.2 Measurement
2.3 Results management
2.4 Method customization
3. Equipment and Consumables Required
This section lists the various devices and consumables required for water tests with the UV5 spectrophotometer and test kits.
4. Tips and Hints
This section contains essential tips and hints for using the test tube holder, cleaning the optical components, and ensuring repeatable measurement results.
5. Appendix - Sample Preparation Procedure
This appendix contains detailed step-by-step instructions for sample preparation for analyzing various water parameters with the corresponding Spectroquant® test kits. The instructions include the required reagents, pH adjustment, mixing procedures, reaction times, and handling of the test tubes or cuvettes.
6. References
This section contains a list of references for further information on water analysis, UV/Vis spectrophotometry, certified reference materials, and LabX® UV/VIS software.
