Bradford Protein Assay - UV Vis Spectroscopy
Spectrophotometric Method for the Bradford Protein Assay of Biological Samples
The Bradford protein assay is a method used in life science applications to determine protein concentration in biological samples spectrophotometrically. For detailed information on the method, equipment required, and results analysis, proceed to download the application note below.
How is the Bradford protein assay determined?
In this assay, Bovine Serum Albumin (BSA) is used as the protein standard. Brilliant Blue G and the protein forms a complex, causing a spectral shift from brown to blue. Samples are measured at a wavelength of 595 nm and a linear curve of absorbance versus concentration of standards is obtained. Using this curve, the concentration of unknown protein samples can be determined.
Other protein assays include the BCA, Lowry and biuret assays. You can find them here:
