This page describes the use of UV Vis spectrophotometry for accurate protein quantification, focusing on the effects of pH on absorbance for tyrosine and tryptophan.
When you download this application package, you will receive a PDF of the application note as well as the required data for the LabX™ UV Vis spectrophotometry software method.
How does UV Vis spectrophotometry work for protein quantification?
UV Vis spectrophotometry measures proteins' absorbance at 280 nm, allowing for accurate concentration determinations based on aromatic amino acid content.
Why is pH important when using UV Vis spectrophotometry for protein analysis?
The pH affects the absorbance characteristics of amino acids, leading to accurate quantification only when using the appropriate buffer solution.
What advantages does UV Vis spectrophotometry offer for protein quantification?
It provides fast and accurate measurements with minimal sample preparation, making it ideal for high-throughput applications in laboratories.
