This application note provides a method for determining the albumin content in blood serum using METTLER TOLEDO's UV/VIS spectrophotometer.
What Is Albumin and Why Is It Important?
Albumin is a water-soluble protein, which is produced in the liver and present in the blood plasma. It plays a crucial role in regulating the amount of fluid in our blood vessels by attracting water and maintaining a balance of pressure within the blood. Albumin is used to treat conditions that result in a loss of blood volume, such as surgery, bleeding, kidney dialysis, or severe injuries. By replenishing lost body fluids, albumin can effectively help to treat low levels of this protein in the blood.
How Is Albumin Determined?
When albumin is reacted with bromocresol green at a slightly acidic pH, albumin triggers a change in the color of the indicator from yellow-green to green-blue. The intensity of the color is measured at a wavelength of 630 nm using a METTLER TOLEDO UV/VIS spectrophotometer to determine the albumin concentration.
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