Guide

Liquid Handling Options for ELISA Protocols

Guide

Tips and Applications to Correctly Perform Enzyme-linked Immunosorbent Assays (ELISAs)

advice from PhD-level cellular and molecular biologists with extensive experience performing ELISAs
advice from PhD-level cellular and molecular biologists with extensive experience performing ELISAs

The ELISA Master Guide discusses how to perform enzyme-linked immunosorbent assays (ELISAs) and provides real-world insights for optimal liquid handling and tool selection.

The guide provides advice from PhD-level cellular and molecular biologists with extensive experience performing ELISAs on how researchers can:

  • Choose the most appropriate ELISA set-up for their research question
  • Carry out successful ELISAs with a range of sample types
  • Improve precision and reproducibility
  • Minimize the risk of inaccurate results

 

This guide is structured into four parts:

  • An overview of the different types of ELISA (direct, indirect, sandwich, competitive and ELISpot assays
  • Factors influencing reproducibility in ELISA protocols: subpar reagent and consumable quality, cross-contamination, pipetting techniques, cross-reactivity, matrix-effect, batch-to-batch reagents and data analysis. For each aspect, the paper provides practical tips for overcoming these challenges and discusses the different types of liquid handling equipment available.
  • Detailed step-by-step instructions on how to perform an ELISA workflow, using a typical sandwich ELISA carried out in a 96-well plate as an example.
  • A discussion of fundamental applications of ELISAs within different areas of research, diagnostics, biopharma, and biotech. This includes real-world examples of ELISA in medical diagnosis, food and environmental testing, drug discovery and development, and quality control.

 

Events

Lab Health and Safety when Pipetting

20 Mar, 2025

In this live webinar, we will give you an overview of risk factors in the lab and measures to minimize these risks when pipetting. more

Pipette Selection Made Easy

27 Mar, 2025

Learn how to evaluate your pipetting needs and discover the perfect solution. Join our live webinar to gain valuable insights into the world of pipetting. more

Live Webinar: Thermal Analysis of Cosmetics

13 Mar, 2025

Discover the science behind beauty! Join our insightful live webinar and delve into essential thermal techniques used to characterize cosmetic products. more