The application note describes a spectroscopic UV method to quantify nicotine in E-Liquids. The method is based on an external calibration using the absorbance of nicotine at 262 nm.
Background
Electronic cigarettes are handheld devices that simulate the feeling of smoking. The so-called E-liquid is vaporized by an electronically heated coil and the vapors are inhaled by the user. The liquid usually consists of propylene glycol, glycerol, flavors and nicotine.
Nicotine is a basic alkaloid that is naturally present in the tobacco plant. It is a psychoactive substance with both stimulant and relaxant effects and therefore an important component of the E-liquids.
Commercially available E-liquids have nicotine concentrations from 3 to 19 mg/mL. It is important to control and maintain the nicotine level in E-liquids to avoid any side effects of usage.
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