Learn more about in-situ Raman spectroscopy with ReactRaman.
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HPLC is a valuable workhorse in your lab, but what really happens between samples?

What is a Raman probe?

raman probe definition

A Raman probe uses laser light to create a "fingerprint" by causing molecules in a sample to vibrate in a specific way when the light hits the sample. The fingerprint is then captured and sent via fiber optic cables to an analyzer, where it is compared to known signals. Raman probes use fibers to deliver the excitation laser beam to the sample and collect the signal, allowing for more flexibility with the sample holder.

These are for immersion, non-contact, and flow. The most common configuration is the immersion probe designed to be inserted into a reactor containing the reaction sample. If the chemistry is corrosive or needs to be sealed, using a non-contact Raman probe is often preferable outside of a site window looking into the reactor. The flow cell can be coupled in-line for flow applications to make continuous measurements.

How do you use a Raman probe?

how to use a raman probe

Connect the SmartConnect™ Raman probe to the spectrometer base unit. Considering the type of sample to be measured, connect the sample interface end of the probe — an immersion probe into a reactor, a flow cell into a bypass loop or flow path, and a non-contact Raman optic at a site glass or window to remotely observe the reaction. Once the connection is complete, use iC Raman software to optimize the data acquisition parameters and set up the experiment profile. 

How does a Raman spectrometer work?

A laser beam from the Raman analyzer is typically directed onto the sample, and the Raman scattered light is collected and returned to the spectrometer.

The resulting Raman spectrum, which represents the intensity of scattered light at different wavelengths, provides information about vibrations within the molecule and can be used to identify substances or study specific molecular interactions.

What is a Raman spectrometer used for?

Raman spectrometers are used for identifying unknown materials and verifying or quantifying known materials. Raman spectroscopy is a non-destructive analysis technique that allows for fast and safe analysis, as no sample preparation is needed and, in some cases, samples can even be analyzed in their original packaging.

Applications of Raman spectroscopy include raw material verification, quality control of incoming goods, identification and analysis of active pharmaceutical ingredients (APIs), additives and excipients, and identification of illicit or counterfeit substances such as drugs.

More applications for laboratory Raman systems:

What is the difference between laboratory and handheld Raman systems?

Handheld Raman systems are typically used for qualitative analysis and identifying unknowns in the field by comparing results against a stored spectral library.

Laboratory Raman systems such as ReactRaman offer better stability, higher resolution, and sensitivity compared to handheld Raman systems. Importantly, with the higher sensitivity of laboratory Raman systems, it is also possible to quantify substances at low concentrations.