Cell and gene therapies have emerged as promising treatments in the medical field, potentially providing life-saving remedies for many diseases. Ensuring the safety and efficacy of these therapies requires quality control measures at every step of the manufacturing process.
UV Vis spectroscopy is a direct analytical technique that can assess cell and gene therapy products' purity, concentration, and stability. By measuring the absorbance of light at specific wavelengths, UV Vis spectroscopy can provide valuable information about the chemical and physical properties of DNA, proteins, viral vectors, and liposomes.
Are you interested in learning more about quality control in gene and cell therapy? Discover how UV Vis spectroscopy can help ensure the safety and efficacy of life-changing treatments by downloading our know-how package on cell and gene therapy containing:
- A white paper about quality control with UV Vis spectroscopy in cell and gene therapy
- A know-how poster about nucleic acid analysis with absorbance spectroscopy
Why Use UV Vis Spectroscopy for DNA Quantification?
UV Vis spectroscopy is a non-destructive analytical method, which means the sample is not damaged during the measurement process. In addition, UV Vis is relatively inexpensive and simple and can provide rapid and accurate information in routine tasks like DNA quantification. The methodology is direct and straightforward. UV Vis is highly versatile. It can be applied for many other applications in the field of cell and gene therapy, such as measuring the stability of DNA (UV melting), measuring protein expression, measuring enzyme activity, or characterizing the bacteria concentration within a cell culture.
Workflow Examples of UV Vis in Cell and Gene Therapy
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Gene Therapy
In gene therapeutic drugs nucleic acids are integrated to a vector or virus. The goal is that the corresponding nucleic acids, inhibit or repair certain cellular processes within the body. DNA quantification with UV Vis is a useful tool during the development of gene therapeutic drugs.
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Cell Therapy
In cell therapy, cells are modified by inserting new genes with a vector or virus. The corresponding gene needs to be synthesized and prepared. UV Vis is a useful tool during this process to ensure the purity of the DNA.
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Oligonucleotide Synthesis
During the synthesis of oligonucleotides (DNA fragments), purity and concentration are crucial parameters. Oligonucleotide synthesis includes many different steps. Every nucleotide coupling needs to be monitored. That is why a fast and simple method such as UV Vis spectroscopy is handy.
How Can Genomic Labs Profit from UV/VIS Excellence Spectrophotometers?
- Intuitive instrument handling with ready-to-use methods and individual shortcuts per user
- Audit trail and electronic signature enable data compliance with regulations such as 21 CFR Part 11
- The instruments need no warm-up time — simply turn on the spectrophotometer and start measuring
- Trustworthy service support including offerings for calibration, user training, and repairs
