Sugar Solution Color using MOPS Buffer - UV Vis Spectroscopy
Method in accordance to ICUMSA™ GS 9 1/2/3-8
This application note is based on the international ICUMSATM method GS 9/1/2/3-8 (2005): "The Determination of Sugar Solution Colour at pH 7.0 by the MOPS Buffer Method". To find out more, please proceed to download the method below.
How is sugar solution color measured spectrophotometrically?
Assessing the color of sugar solution is one of the most important quality markers for sugar producers and refiners. This method is applicable for plantation white sugars, raw sugars and very low-color sugars. It is designed for all sugars in the solution color value up to 16000 ICUMSATM Units at pH 7.0 (IU7.0).
The absorbance value of sugars are pH dependent. The absorbance increases when the pH value is increased and vice versa. Therefore, prior to the color determination, the pH is measured and adjusted to neutral using MOPS Buffer.
MOPS (3-(N-morpholino)propanesulfonic acid) is an excellent buffer for many systems at near-neutral pH.
The UV5 Excellence spectrophotometer gives the direct result in terms of the ICUMSATM value, using the predefined method that comes with the instrument. Download the application for complete equipment, method and results information.
* ICUMSA® is a trademark of ICUMSA Ltd. and Verlag Dr. Albert Bartens KG.
