Glucose Measurement - UV Vis Spectroscopy

This application note describes how to determine the glucose content in wine using a sample and reagent kit along with a UV/VIS Excellence Spectrophotometer. To find out more, please proceed to download the application note below.

Why does the amount of glucose in wine need to be measured?

Glucose measurement is an important part of the wine production process as the amount of sugar in wine is indicative of the final alcohol content. Glucose, one of the primary sugars found in wine grapes, is a six-carbon sugar molecule derived from the breakdown of sucrose. These sugars are broken down during the fermentation process to form ethanol and carbon dioxide.

How is glucose measurement conducted?

In this experiment, a kit for the quantitative, enzymatic determination of glucose in wine and other materials is used in conjunction with the UV7 Excellence Spectrophotometer from METTLER TOLEDO. A spectrophotometer is a popular glucose measurement device due to its speed and ease of use. The absorbance of standard and sample solutions are both measured at 340nm. The difference in absorbance is directly proportional to the concentration of glucose in wine.