UV/Vis Spectrophotometry

Spectrophotometry is the measurement of light absorption by a substance as a function of wavelength. It is commonly used in analytical chemistry, biochemistry, and molecular biology.

UV Vis spectrophotometry can be used to determine the concentration of a solution, to identify and quantify the components of a mixture, and to study the kinetics of a reaction.

Find our more in the UV Vis Application Library

Spectroscopy is a broader term that refers to the study of the interaction between electromagnetic radiation and matter. Spectrophotometry is a specific type of spectroscopy that measures the absorption of light by a substance.

Learn more about the theory of UV Vis spectrophotometry in the Easy Spectrophotometry Guide or the Spectrophotometry Applications and Fundamentals Guide

A UV Vis spectrophotometer measures the amount of light absorbed by a sample at a specific wavelength. The amount of light absorbed is directly proportional to the concentration of the substance in the sample.

UV Vis spectrophotometry is a fast, accurate, and non-destructive method of analyzing samples. It can be used for a wide range of substances and is widely accepted as a standard analytical technique in many industries.

UV Vis spectrophotometry is used in the pharmaceutical industry to analyze the purity, identity, and strength of drugs. It can also be used to monitor the degradation of drugs over time.

To learn more about how spectrophotometry can ensure therapeutic quality, read our customer story

A blank is a sample that contains all the reagents except the substance being tested. It is used to calibrate the spectrophotometer and to correct for any background absorption or interference.

Yes, UV Vis spectrophotometry can be used to analyze biological samples such as proteins, nucleic acids, and enzymes. It can also be used to quantify the amount of a particular compound in a biological sample.

Learn more in the Life Science Toolbox for UV Vis Spectrophotometry

To ensure accuracy, it is important to properly calibrate the spectrophotometer, use appropriate reagents and standards, and follow a standardized protocol for sample preparation and measurement. It is also important to validate your results by comparing them to other methods of analysis.

Find out more about the METTLER TOLEDO service offerings for UV Vis calibration